• Comprehensive: Outstanding performance for small, medium and large proteins
• Convenience: Simultaneously transfer two mini gels
• Compatible: Highly compatible with different types of precast or homemade gels
• Simple: Easy to assemble, one button operation and customizable programs available
Comparative blotting efficiency with various transfer systems.
Three different proteins were transferred on to PVDF membranes using traditional wet transfer, iBlot, Trans-Blot, and GenScript's eBlot L1. The quantities of proteins loaded ranged from 1.25 µg- 20 µg for EGFR, 7.8 ng- 125 ng for Taq, and 0.6 µg- 10µg for GAPDH. For all three proteins, the eBlot® L1 offered superior transfer efficiency compared to other systems. With its high efficiency of transfer, protein concentrations as low as 7 ng for Taq (lane 5), and as high as 20 µg for EGFR(lane 1) could be clearly visualized.