細胞凋亡偵測套組_ Annexin V Detection Kit-盟基生物科技股份有限公司

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細胞凋亡偵測套組_ Annexin V Detection Kit

細胞凋亡偵測套組_ Annexin V Detection Kit
套組買大包裝,優惠獎不完!

優惠促銷方案請洽當區業務代表,或來電洽詢。


一、細胞凋亡
細胞凋亡的發生是多重事件進行一連串的訊號傳遞所產生的現象,提供許多機會(表徵)予研究者來評估其中的過程。
細胞凋亡前期,細胞膜不對稱性(asymmetry)的喪失伴隨發生的是:原本鑲嵌於細胞質膜(plasma membrane)的PS (phosphatidylserine)基團逐漸外翻露出膜外。
Annexin V 則是一種鈣離子依賴型磷脂結合蛋白,對PS具有高度親和力。因此,透過流式細胞儀(flow cytometry)輔以Annexin V鍵結的螢光染劑普遍被應用於偵測早期階段的細胞凋亡。

 


二、Annexin V Apoptosis Detection Kit
一定要告訴您的六大優勢!找盟基,準沒錯!

1. 多樣螢光選擇:15種螢光*3種核酸染劑
2. 高精準度:應用Flow cytometry偵測早期細胞凋亡的最佳指標(Annexin V ),
3. 即時偵測,杜絕細胞固定造成的偽陽性(false positive)
4. 操作快又簡單:僅需15-20分鐘(hand-on時間)
5. 多元化包裝:20, 50, 100, 200 assays
6. 嚴謹的品管:Elabscience自家生產,非ODM/OEM代工。原料可追朔,高品質值得信賴。



訂購資訊:偵測套組  雙重驗證Apoptosis!
貨號 品名 Annexin V 螢光 Ex/Em 包裝
E-CK-A217 Annexin V-APC / PI Apoptosis Detection Kit APC: 650 / 660 nm 20 /50 / 100/ 200 Assays
E-CK-A218 Annexin V-APC / 7-AAD Apoptosis Detection Kit
E-CK-A258 Annexin V-APC / DAPI Apoptosis Detection Kit
E-CK-A229 Annexin V-APC/Cyanine7 / PI Apoptosis Detection Kit APC/Cyanine7: 650;760 / 780 nm
E-CK-A230 Annexin V-APC/Cyanine7 / 7-AAD Apoptosis Detection Kit
E-CK-A270 Annexin V-APC/Cyanine7 / DAPI Apoptosis Detection Kit
E-CK-A237 Annexin V-AF488 / PI Apoptosis Detection Kit AF488: 495 / 520 nm
E-CK-A238 Annexin V-AF488 / 7-AAD Apoptosis Detection Kit
E-CK-A278 Annexin V-AF488 / DAPI Apoptosis Detection Kit
E-CK-A213 Annexin V-AF647 / PI Apoptosis Detection Kit AF647: 650 / 670 nm
E-CK-A214 Annexin V-AF647 / 7-AAD Apoptosis Detection Kit
E-CK-A254 Annexin V-AF647 / DAPI Apoptosis Detection Kit
E-CK-A221 Annexin V-Cyanine5 / PI Apoptosis Detection Kit Cyanine5: 650 / 670 nm
E-CK-A222 Annexin V-Cyanine5 / 7-AAD Apoptosis Detection Kit
E-CK-A262 Annexin V-Cyanine5 / DAPI Apoptosis Detection Kit
E-CK-A233 Annexin V-EV450 / PI Apoptosis Detection Kit EV450: 410 / 450 nm
E-CK-A234 Annexin V-EV450 / 7-AAD Apoptosis Detection Kit
E-CK-A235 Annexin V-EV500 / PI Apoptosis Detection Kit EV500: 425 / 500 nm
E-CK-A236 Annexin V-EV500 / 7-AAD Apoptosis Detection Kit
E-CK-A239 Annexin V-ER780 / PI Apoptosis Detection Kit ER780: 650;760 / 780 nm
E-CK-A240 Annexin V-ER780 / 7-AAD Apoptosis Detection Kit
E-CK-A280 Annexin V-ER780 / DAPI Apoptosis Detection Kit
E-CK-A219 Annexin V-EGFP / PI Apoptosis Detection Kit EGFP: 488 / 510 nm
E-CK-A220 Annexin V-EGFP / 7-AAD Apoptosis Detection Kit
E-CK-A260 Annexin V-EGFP / DAPI Apoptosis Detection Kit
E-CK-A211 Annexin V-FITC / PI Apoptosis Detection Kit FITC: 490 / 530 nm
E-CK-A212 Annexin V-FITC / 7-AAD Apoptosis Detection Kit
E-CK-A252 Annexin V-FITC / DAPI Apoptosis Detection Kit
E-CK-A216 Annexin V-PE / 7-AAD Apoptosis Detection Kit PE: 495;565 / 575 nm
E-CK-A256 Annexin V-PE / DAPI Apoptosis Detection Kit
E-CK-A264 Annexin V-PE/Cyanine5 / DAPI Apoptosis Detection Kit PE/Cyanine5: 495;565;655 / 670 nm
E-CK-A266 Annexin V-PE/Cyanine5.5 / DAPI Apoptosis Detection Kit PE/Cyanine5.5: 495;565 / 690 nm
E-CK-A228 Annexin V-PE/Cyanine7 / 7-AAD Apoptosis Detection Kit PE/Cyanine7: 495;565;755 / 775 nm
E-CK-A268 Annexin V-PE/Cyanine7 / DAPI Apoptosis Detection Kit
E-CK-A272 Annexin V-PE/TR / DAPI Apoptosis Detection Kit DAPI 405/488; 454nm
PE/TR 488/495; 565nm


三、Annexin V Apoptosis Detection / Dead cell stain Reagent
分開單獨偵測可以,自行搭配試劑套組也沒問題!!

實例驗證:搭配不同螢光,偵測依然準確犀利!
Annexin V-EGFP + PI Annexin V-PE/Cyanine5 / DAPI  
Jurkat cells were cultured with (Left) or without (Right) 5 μM Camptothecin for 4 h. Annexin V-EGFP single-positive cells were early apoptotic cells, Annexin V-EGFP /PI or Annexin V-PE/Cyanine5 / DAPI  
double-positive cells were necrotic or late apoptotic cells, and PI single-positive cells were naked nuclei.


訂購資訊:相關染劑及試劑
類別 貨號 品名 Annexin V 螢光 Ex/Em 包裝
Annexin V staining E-CK-A117 Annexin V-APC Reagent APC: 650 / 660 nm 50/100/200/500 Tests
E-CK-A129 Annexin V-APC/Cyanine7 Reagent APC/Cyanine7: 650;760 / 780 nm 50/100/200/500 Tests
E-CK-A137 Annexin V-AF488 Reagent AF488: 495 / 520 nm 50/100/200/500 Tests
E-CK-A137U Annexin V-AF488 Azide-Free Lyophilized Powder 25ug
E-CK-A113 Annexin V-AF647 Reagent AF647: 650 / 670 nm 50/100/200/500 Tests
E-CK-A113U Annexin V-AF647 Azide-Free Lyophilized Powder 25ug
E-CK-A110 Annexin V-Biotin Reagent X 50/100/200/500 Tests
E-CK-A110U Annexin V-Biotin Azide-Free Lyophilized Powder 25ug
E-CK-A121 Annexin V-Cyanine5 Reagent Cyanine5: 650 / 670 nm 50/100/200/500 Tests
E-CK-A133 Annexin V-EV450 Reagent EV450: 410 / 450 nm 50/100/200/500 Tests
E-CK-A133U Annexin V-EV450 Azide-Free Lyophilized Powder 25ug
E-CK-A135 Annexin V-EV500 Reagent EV500: 425 / 500 nm 50/100/200/500 Tests
E-CK-A135U Annexin V-EV500 Azide-Free Lyophilized Powder 25ug
E-CK-A139 Annexin V-ER780 Reagent ER780: 650;760 / 780 nm 50/100/200/500 Tests
E-CK-A119 Annexin V-EGFP Reagent EGFP: 488 / 510 nm 50/100/200/500 Tests
E-CK-A119U Annexin V-EGFP Azide-Free Lyophilized Powder 25ug
E-CK-A111 Annexin V-FITC Reagent FITC: 490 / 530 nm 50/100/200/500 Tests
E-CK-A111U Annexin V-FITC Azide-Free Lyophilized Powder 25ug
E-CK-A115 Annexin V-PE Reagent PE: 495;565 / 575 nm 50/100/200/500 Tests
E-CK-A123 Annexin V-PE/Cyanine5 Reagent PE/Cyanine5: 495;565;655 / 670 nm 50/100/200/500 Tests
E-CK-A125 Annexin V-PE/Cyanine5.5 Reagent PE/Cyanine5.5: 495;565 / 690 nm 50/100/200/500 Tests
E-CK-A127 Annexin V-PE/Cyanine7 Reagent PE/Cyanine7: 495;565;755 / 775 nm 50/100/200/500 Tests
E-CK-A131 Annexin V-PE/TR Reagent PE/TR 488/495; 565nm 50/100/200/500 Tests
DNA staining E-CK-A161 Propidium Iodide (PI) Staining Solution PI: 546 / 617 nm 50/100/200/500 Tests
E-CK-A162 7-AAD Viability Staining Solution 7-AAD: 545 / 647 nm 50/100/200/500 Tests
E-CK-A163 DAPI Reagent DAPI: 350 / 470nm 50/100/200/500 Tests
Buffer E-CK-A151 Annexin V Binding Buffer (10X) X 10mL


四、更多Apoptosis相關實驗試劑,請來電或洽詢當區業務
方法 Annexin V apoptosis assay Caspase assay Mitochondrial membrane potential assay (JC-1) TUNEL assay
偵測目標 Phosphatidylserine (PS) Caspase activity Mitochondrial membrane potential Fragmented DNA
凋亡階段 Early → Late
偵測方法 Flow cytometry ELISA reader (OD) Flow cytometry, Fluorescence microscopy Fluorescence microscopy
反應時間 15-20 mins 2-4 hours 15-20 mins 90-100 mins