Customized Mouse Monoclonal Antibody -盟基生物科技股份有限公司

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Customized Mouse Monoclonal Antibody

antibody development

Customized Mouse Monoclonal Antibody

antibody development

Customized Mouse Monoclonal Antibody

客製化鼠單株抗體

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一、服務介紹

小鼠單株抗體具有特異性高、親和性高、遺傳穩定等優點，應用於科學研究、醫學診斷、生物醫學等領域。
Elabscience已成功為客戶完成近3000個專案服務。經過技術平臺的全面升級，在抗體的驗證和下游應用方面為客戶提供更多元的客製化服務。

二、服務優勢

1. 高品質的蛋白抗原生產（同時具備原核蛋白和真核蛋白表達平臺）
2. 針對不同應用可提供40株陽性細胞株上清液供客戶篩選驗證
3. 滿足客戶各種驗證和篩選的需求，確保抗體特異性
4. 可提供親和純化的單克隆抗體3-10mg及對應融合瘤細胞株
5. 可提供WB、IHC、IF、FACS、中和功能等全面的抗體驗證服務

三、服務方案
1.流程

2.方案

服務流程	到貨規格	製作週期
1. Project analysis and antigen design
1-1. Project analysis and antigen design	Project analysis report	2 days
2. Protein production
2-1. Gene synthesis and Codon optimization	100µg protein sample (>80% purity) Expression analysis report	4-6 weeks
2-2. Carrier construction
2-3. Transfection and protein Expression Evaluation
2-4. Scale up protein expression and purification
2-5. SDS-PAGE analysis
3. Immunization and Titer test
3-1. Immunize 5 mice	ELISA test report (ELISA titer>1:64,000)	6-9 weeks
3-2. Standard immune schedule includes 5 immunization
3-3. Indirect ELISA test
4. Hybridoma fusion
4-1. Hybridoma fusion	Project report	1-2 weeks
5. Screening and subcloning
5-1. Positive clones screening	500µL supernatant per clone, about 10 clones Project report Hybridoma clone (1 vial of each clone)	3-4 weeks
5-2. 2 rounds of subcloning
6. Antibody production & purification - Optional
6-1. Hybridoma culture	Antibody sample (3-10mg/clone, liquid) Hybridoma cells (2 vials of each clone)	3-4 weeks
6-2. Inject the hybridoma cells into mice to prepare ascites
6-3. Purify the antibody from the ascites

四、客戶提供資料

1. 抗原資訊(名稱、序列)
2. 是否需要代為生產抗原
3. 後續應用
4. 抗體純化方式(Protein A/G或Antigen affinity purification)

五、到貨規格

1. 結案報告
2. 500uL克隆上清液
3. 融合瘤細胞株(1~2株)
4. 100ug蛋白(如有委託生產抗原)
5. 3-10mg抗體(如有委託純化抗體)


Mouse anti-SETD3 mAb published in Cell Death Dis. 2019;10(3):183.	Mouse anti-P. gingivalis mAb published in ONCOLOGY LETTERS 21: 189, 2021.

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