ELISA (酵素連結免疫吸附分析法)-Assay Kit-盟基生物科技股份有限公司

Assay Kit

ELISA (酵素連結免疫吸附分析法)

ELISA (酵素連結免疫吸附分析法)

Assay Kit

ELISA (酵素連結免疫吸附分析法)

高品質高穩定的 ELISA 套組,高精準度、高靈敏度、價格划算。

蛋白質實驗中最常使用 Western blotting 偵測目標蛋白質,但是此方法每次實驗的樣本數量有限,且無法精準地定量。因此,針對多樣本和定量的需求,一般會使用 Enzyme-linked immunosorbent assay (ELISA) (酵素連結免疫吸附分析法)。和 Western blotting 一樣,ELISA 也是透過抗體和抗原之間的專一性,辨認樣本中的目標蛋白質或小分子。經過樣本吸附和沖洗的過程,最後由抗體上面帶的 HRP 酵素,和 HRP 基質反應 (通常使用 TMB),最後偵測吸光值。對照標準品和樣本的呈色深淺,可以推算出目標蛋白質或小分子的濃度。

一、產品介紹
Elabscience 提供超過一千種 ELISA 套組選擇,針對多種物種和不同訊息功能,可以用來檢測細胞或組織中的蛋白質表現量或小分子含量。同時,Elabscience 嚴格的品質監控和優良的線性區間,使得其榮登2019 CiteAb Awards,年度最值得推薦 ELISA kit 供應商
除了一般 ELISA 吸光值偵測方法之外,Elabscience 還提供 CLIA kit (Chemiluminescent Immunoassay)。CLIA 和 ELISA,中間操作步驟完全相同,差別只在最後一步 HRP 基質,CLIA 使用冷光 HRP 基質,靈敏度更升級,為傳統 ELISA 的5倍以上,可到10 pg/mL 等級 (表一)。CLIA 有超過600種產品,任君挑選。

表一:CLIA 靈敏度高於一般 ELISA,下方以 Mouse TFF3 舉例
  ELISA CLIA
貨號 E-EL-M1200 E-CL-M0666
偵測目標 Mouse TFF3(Trefoil Factor 3, Intestinal)
反應時間 3.5h
偵測方式 吸光值 (A450) 冷光 (RLU)
靈敏度 46.88 pg/mL 9.38 pg/mL
終止反應 需加入 stop solution 不需要


二、產品優點
1. 產品眾多:
針對多種物種,包括 human, mouse, rat, rabbit, monkey, porcine 等。
針對多種功能,包括 Cancer, Cardiovascular, Cell Biology, Cytokines, Developmental Biology, Epigenetics and Nuclear Signal, Immunology, Metabolism. Microbiology, Neuroscience, Signal Transduction, Stem Cells, Tags and Cell Markers 等。


2. 直接使用最方便:
包含所有試劑,盤子 (pre-coated)、抗體、清洗溶液、標準品等,不須額外準備和購買試劑。其中盤子為 pre-coated (預先吸附抗體),確保其產品穩定性 (Elabscience 不販售 uncoated ELISA)。

3. 高精準度:
Inter CV% 和 Intra CV% 皆小於10%,代表同一盤和不同盤之間的誤差極小,使得實驗重複性高,實驗數據更有說服力。

4. 高靈敏度:
靈敏度高,可到 pg 等級,也可選擇 CLIA kit (Chemiluminescent Immunoassay) 款式,靈敏度更升級 (表一)。

5. 高物種專一性:
不同物種之間的交互作用 Cross reactivity<10%。

6. 多篇文獻引用:
Elabscience 高品質的 ELISA 套組深受實驗者喜愛,已超過2000篇以上引用文獻,包含 Nature Medicine, Circulation, Cell Research, Gastroenterology 等,引用資訊請點我

7. 品質嚴謹:
100%自行研發與製造,全部資料可追溯。

8. 包裝彈性:
可選擇24或96反應數包裝,需要更大量可客製化訂做,提供更大量包裝,價格更優惠。


三、產品資訊
超過一千種產品,以下列出熱銷排行榜前100名產品,全部產品列表請點選此處連結
產品僅能儲存於4℃一個月,若預估未能於一個月內使用完畢,請於收到產品後立即拆封,並將 ELISA Plate, Standard, Concentrated Biotinylated Detection Ab (100×), Concentrated HRP Conjugate (100×) 儲存於-20℃,其他試劑儲存於4℃,如此可延長保存期限至少半年。

表二:熱銷排行榜前100名產品列表
針對目標 貨號 產品名稱 方法 針對物種 線性區間
8-epi-PGF2α E-EL-0041 8-epi-PGF2α(8-Epi-Prostaglandin F2 Alpha) ELISA Kit Competitive Universal 15.63~1000pg/mL
8-OHdG E-EL-0028 8-OHdG(8-Hydroxydeoxyguanosine) ELISA Kit Competitive Universal 1.56~100ng/mL
ACH E-EL-0081 ACH(Acetylcholine) ELISA Kit Competitive Universal 15.63~1000pg/mL
ACTH E-EL-R0048 Rat ACTH(Adrenocorticotropic Hormone) ELISA Kit Competitive Rat 15.63~1000pg/mL
ADMA E-EL-0042 ADMA(Asymmetrical Dimethylarginine) ELISA Kit Competitive Universal 15.63~1000ng/mL
ADP/Acrp30 E-EL-H6122 Human ADP/Acrp30(Adiponectin) ELISA Kit Sandwich Human 0.16~10ng/mL
Ang-Ⅱ E-EL-H0326 Human Ang-Ⅱ(Angiotensin Ⅱ) ELISA Kit Competitive Human 31.25~2000pg/mL
APLN E-EL-H0456 Human APLN(Apelin) ELISA Kit Competitive Human 62.5~4000pg/mL
ApoB48 E-EL-H6084 Human ApoB48(Apolipoprotein B48) ELISA Kit Sandwich Human 1.56~100ng/mL
Aβ1-40 E-EL-H0542 Human Aβ1-40(Amyloid Beta 1-40) ELISA Kit Sandwich Human 15.63~1000pg/mL
BDNF E-EL-H0010 Human BDNF(Brain Derived Neurotrophic Factor) ELISA Kit Sandwich Human 31.25~2000pg/mL
C3a E-EL-M0337 Mouse C3a(Complement Component 3a) ELISA Kit Sandwich Mouse 0.156~10ng/mL
C5a E-EL-MK1612 Monkey C5a(Complement Component 5a) ELISA Kit Sandwich Monkey 0.16~10ng/mL
CPP E-EL-H0851 Human CPP(Copeptin) ELISA Kit Competitive Human 31.25~2000pg/mL
CRP E-EL-H0043 Human CRP(C-Reactive Protein) ELISA Kit Sandwich Human 0.39~25ng/mL
CTXⅠ E-EL-H0835 Human CTXⅠ(Cross Linked C-telopeptide of Type Ⅰ Collagen) ELISA Kit Sandwich Human 0.16~10ng/mL
CTXⅠ E-EL-M3023 Mouse CTXⅠ(Cross Linked C-telopeptide of Type Ⅰ Collagen) ELISA Kit Sandwich Mouse 31.25~2000pg/mL
CTXⅠ E-EL-R1456 Rat CTXⅠ(Cross Linked C-telopeptide of Type Ⅰ Collagen) ELISA Kit Sandwich Rat 0.16~10ng/mL
CTX-Ⅱ E-EL-H0837 Human CTX-Ⅱ(Cross Linked C-telopeptide of Type Ⅱ Collagen) ELISA Kit Sandwich Human 0.16~10ng/mL
DA E-EL-0046 DA(Dopamine) ELISA Kit Competitive Universal 31.25~2000pg/mL
E2 E-EL-0150 Human/Monkey/Mouse E2(Estradiol) Competitive Human/Monkey/Mouse 9.37~600pg/mL
E2 E-EL-0152 Rat/Porcine E2(Estradiol) ELISA Kit Competitive Rat/Porcine 1.56~100pg/mL
EPI E-EL-0045 EPI(Epinephrine/Adrenaline) ELISA Kit Competitive Universal 31.25~2000pg/mL
ET-1 E-EL-H0064 Human ET-1(Endothelin 1) ELISA Kit Sandwich Human 1.25~80pg/mL
F1+2 E-EL-H1793 Human F1+2(Prothrombin Fragment 1+2) ELISA Kit Sandwich Human 46.88~3000pg/mL
FGF21 E-EL-H0074 Human FGF21(Fibroblast Growth Factor 21) ELISA Kit Sandwich Human 31.25~2000pg/mL
FGF23 E-EL-H1116 Human FGF23(Fibroblast Growth Factor 23) ELISA Kit Sandwich Human 15.63~1000pg/mL
FNDC5 E-EL-H2254 Human FNDC5(Fibronectin type Ⅲ domain-containing protein 5) ELISA Kit Sandwich Human 0.16~10ng/mL
FSH E-EL-R0391 Rat FSH(Follicle Stimulating Hormone) ELISA Kit Sandwich Rat 3.13~200ng/mL
GAL3 E-EL-H1470 Human GAL3(Galectin 3) ELISA Kit Sandwich Human 0.16~10ng/mL
GFAP E-EL-H6093 Human GFAP(Glial Fibrillary Acidic Protein) ELISA Kit Sandwich Human 15.63~1000pg/mL
GHRL E-EL-H1919 Human GHRL(Ghrelin) ELISA Kit Competitive Human 0.16~10ng/mL
GLP-1 E-EL-H6025 Human GLP-1(Glucagon Like Peptide 1) ELISA Kit Sandwich Human 1.56~100pg/mL
GPX1 E-EL-H5410 Human GPX1(Glutathione Peroxidase 1) ELISA Kit Sandwich Human 31.25~2000pg/mL
GSH E-EL-0026 GSH(Glutathione) ELISA Kit Competitive Universal 1.56~100μg/mL
HBsAg E-EL-H6080 Human HBsAg(Hepatitis B surface antigen) ELISA Kit Sandwich Human 0.31~20ng/mL
Hepc E-EL-H6013 Human Hepc(Hepcidin) ELISA Kit Sandwich Human 62.5~4000pg/mL
HIF-1α E-EL-H6066 Human HIF-1α(Hypoxia Inducible Factor 1 Alpha) ELISA Kit Sandwich Human 62.5~4000pg/mL
HIS E-EL-0032 HIS(Histamine) ELISA Kit Competitive Universal 1.56~100ng/mL
HMGB-1 E-EL-H1554 Human HMGB-1(High Mobility Group Protein B1) ELISA Kit Sandwich Human 31.25~2000pg/mL
HMGB-1 E-EL-M0676 Mouse HMGB-1(High Mobility Group Protein B1) ELISA Kit Sandwich Mouse 15.625~1000pg/mL
HS E-EL-H2364 Human HS(Heparan Sulfate) ELISA Kit Sandwich Human 0.31~20ng/mL
hs-CRP E-EL-H5134 Human hs-CRP(high-sensitivity C-Reactive Protein) ELISA Kit Sandwich Human 15.63~1000pg/mL
IDO E-EL-H2162 Human IDO(Indoleamine-2,3-Dioxygenase) ELISA Kit Sandwich Human 0.31~20ng/mL
IFN-γ E-EL-H0108 Human IFN-γ(Interferon Gamma) ELISA Kit Sandwich Human 15.63~1000pg/mL
IGF-1 E-EL-H0086 Human IGF-1(Insulin-like Growth Factor 1) ELISA Kit Sandwich Human 1.56~100ng/mL
IL-10 E-EL-H0103 Human IL-10(Interleukin 10) ELISA Kit Sandwich Human 7.81~500pg/mL
IL-10 E-EL-R0016 Rat IL-10(Interleukin 10) ELISA Kit Sandwich Rat 31.25~2000pg/mL
IL-17 E-EL-H0105 Human IL-17(Interleukin 17) ELISA Kit Sandwich Human 31.25~2000pg/mL
IL-1β E-EL-R0012 Rat IL-1β(Interleukin 1 Beta) ELISA Kit Sandwich Rat 31.25~2000pg/mL
IL-1β E-EL-H0149 Human IL-1β(Interleukin 1 Beta) ELISA Kit Sandwich Human 7.81~500pg/mL
IL-1β E-EL-M0037 Mouse IL-1β(Interleukin 1 Beta) ELISA Kit Sandwich Mouse 7.81~500pg/mL
IL-23 E-EL-H0107 Human IL-23(Interleukin 23) ELISA Kit Sandwich Human 39.06~2500pg/mL
IL-35 E-EL-H2443 Human IL-35(Interleukin 35) ELISA Kit Sandwich Human 15.63~1000pg/mL
IL-6 E-EL-H0102 Human IL-6(Interleukin 6) ELISA Kit Sandwich Human 7.81~500pg/mL
IL-6 E-EL-R0015 Rat IL-6(Interleukin 6) ELISA Kit Sandwich Rat 12.5~800pg/mL
IL-6 E-EL-M0044 Mouse IL-6(Interleukin 6) ELISA Kit Sandwich Mouse 31.25~2000pg/mL
IL-8 E-EL-H6008 Human IL-8(Interleukin 8) ELISA Kit Sandwich Human 7.81~500pg/mL
INS E-EL-R3034 Rat INS(Insulin) ELISA Kit Sandwich Rat 6.25~400pg/mL
IP3 E-EL-0059 IP3(Inositol Triphosphate) ELISA Kit Competitive Universal 15.63~1000pg/mL
KL E-EL-H5451 Human KL(Klotho) ELISA Kit Sandwich Human 0.31~20ng/mL
LEP E-EL-H6017 Human LEP(Leptin) ELISA Kit Sandwich Human 15.63~1000pg/mL
LEP E-EL-R0582 Rat LEP(Leptin) ELISA Kit Sandwich Rat 0.16~10ng/mL
LH E-EL-R0026 Rat LH(Luteinizing Hormone) ELISA Kit Sandwich Rat 1.56~100mIU/mL
LH E-EL-M3053 Mouse LH(Luteinizing Hormone) ELISA Kit Sandwich Mouse 0.31~20ng/mL
LL-37 E-EL-H2438 Human LL-37(Antibacterial Protein LL-37) ELISA Kit Sandwich Human 1.56~100ng/mL
MCP-1 E-EL-H6005 Human MCP-1(Monocyte Chemotactic Protein 1) ELISA Kit Sandwich Human 62.5~4000pg/mL
MDA E-EL-0060 MDA(Malondialdehyde) ELISA Kit Competitive Universal 31.25~2000ng/mL
MR-ProADM E-EL-H5441 Human MR-ProADM(Mid-regional pro-adrenomedullin) ELISAKit Sandwich Human 15.63~1000pg/mL
MT E-EL-H2016 Human MT(Melatonin) ELISA Kit Competitive Human 15.63~1000pg/mL
NA/NE E-EL-0047 NA/NE(Noradrenaline/Norepinephrine) ELISA Kit Competitive Universal 0.31~20ng/mL
NGAL E-EL-H0096 Human NGAL(Neutrophil Gelatinase Associated Lipocalin) ELISA Kit Sandwich Human 0.16~10ng/mL
NT-proBNP E-EL-M0834 Mouse NT-proBNP(N-terminal pro-Brain Natriuretic Peptide) ELISA Kit Sandwich Mouse 15.625~1000pg/mL
NT-proBNP E-EL-H0902 Human NT-proBNP(N-Terminal Pro-Brain Natriuretic Peptide) ELISA Kit Sandwich Human 0.63~40ng/mL
OC/BGP E-EL-R0243 Rat OC/BGP(Osteocalcin) ELISA Kit Sandwich Rat 0.78~50ng/mL
OC/BGP E-EL-M0864 Mouse OC/BGP(Osteocalcin) ELISA Kit Sandwich Mouse 0.391~25ng/mL
OPG E-EL-H1341 Human OPG(Osteoprotegerin) ELISA Kit Sandwich Human 0.16~10ng/mL
OT E-EL-0029 OT(Oxytocin) ELISA Kit Competitive Universal 15.63~1000pg/mL
OxLDL E-EL-H6021 Human OxLDL(Oxidized Low Density Lipoprotein) ELISA Kit Sandwich Human 62.5~4000pg/mL
PⅠNP E-EL-H0185 Human PⅠNP(Procollagen Ⅰ N-Terminal Propeptide) ELISA Kit Sandwich Human 15.63~1000pg/mL
PⅠNP E-EL-M0233 Mouse PⅠNP(Procollagen Ⅰ N-Terminal Propeptide) ELISA Kit Sandwich Mouse 93.75~6000pg/mL
PⅢNP E-EL-H0183 Human PⅢNP(N-Terminal Procollagen Ⅲ Propeptide) ELISA Kit Sandwich Human 23.44~1500pg/mL
PAP E-EL-H2101 Human PAP(Plasmin-Antiplasmin Complex) ELISA Kit Sandwich Human 3.13~200ng/mL
PGE2 E-EL-0034 PGE2(Prostaglandin E2) ELISA Kit Competitive Universal 31.25~2000pg/mL
PKM2 E-EL-H6063 Human PKM2(Pyruvate Kinase, Muscle) ELISA Kit Sandwich Human 15.63~1000IU/mL
PYY E-EL-H1237 Human PYY(Peptide YY) ELISA Kit Sandwich Human 31.25~2000pg/mL
S100B E-EL-H1297 Human S100B(S100 Calcium Binding Protein B) ELISA Kit Sandwich Human 31.25~2000pg/mL
SOD1 E-EL-H1113 Human SOD1(Superoxide Dismutase 1, Soluble) ELISA Kit Competitive Human 62.5~4000pg/mL
ST/5-HT E-EL-0033 ST/5-HT(Serotonin/5-Hydroxytryptamine) ELISA Kit Competitive Universal 15.63~1000ng/mL
TGF-β1 E-EL-H0110 Human TGF-β1(Transforming Growth Factor Beta 1) ELISA Kit Sandwich Human 31.25~2000pg/mL
TIMP-1 E-EL-H0184 Human TIMP-1(Tissue Inhibitors of Metalloproteinase 1) ELISA Kit Sandwich Human 0.16~10ng/mL
TL1A E-EL-MK1946 Monkey TL1A(Tumor Necrosis Factor Related Ligand 1A) ELISA Kit Sandwich Monkey 0.16~10ng/mL
TNF-α E-EL-H0109 Human TNF-α(Tumor Necrosis Factor Alpha) ELISA Kit Sandwich Human 7.81~500pg/mL
TNF-α E-EL-R0019 Rat TNF-α(Tumor Necrosis Factor Alpha) ELISA Kit Sandwich Rat 78.13~5000pg/mL
TNF-α E-EL-M0049 Mouse TNF-α(Tumor Necrosis Factor Alpha) ELISA Kit Sandwich Mouse 31.25~2000pg/mL
TSH E-EL-R0976 Rat TSH(Thyroid Stimulating Hormone) ELISA Kit Sandwich Rat 1.25~80ng/mL
VEGF-A E-EL-H0111 Human VEGF-A(Vascular Endothelial Cell Growth Factor A) ELISA Kit Sandwich Human 31.25~2000pg/mL
VF E-EL-H1763 Human VF(Visfatin) ELISA Kit Sandwich Human 0.31~20ng/mL
Zonulin E-EL-H5560 Human Zonulin ELISA Kit Sandwich Human 0.78~50ng/mL
β-EP E-EL-H0572 Human β-EP(Beta-Endorphin) ELISA Kit Competitive Human 15.63~1000pg/mL